Loading and pre-loading processes generate a distinct siRNA population in Tetrahymena

Loading and pre-loading processes generate a distinct siRNA population in Tetrahymena☆

The various properties of small RNAs, such as length, terminal nucleotide, thermodynamic asymmetry and duplex mismatches, can impact their sorting into different Argonaute proteins in diverse eukaryotes. The developmentally regulated 26- to 32-nt siRNAs (scnRNAs) are loaded to the Argonaute protein Twi1p and display a strong bias for uracil at the 5' end. In this study, we used deep sequencing ...

A Tetrahymena Hsp90 co-chaperone promotes siRNA loading by ATP-dependent and ATP-independent mechanisms

The loading of small interfering RNAs (siRNAs) and microRNAs into Argonaute proteins is enhanced by Hsp90 and ATP in diverse eukaryotes. However, whether this loading also occurs independently of Hsp90 and ATP remains unclear. We show that the Tetrahymena Hsp90 co-chaperone Coi12p promotes siRNA loading into the Argonaute protein Twi1p in both ATP-dependent and ATP-independent manners in vitro....

Extraction-Transformation-Loading Processes

A data warehouse (DW) is a collection of technologies aimed at enabling the knowledge worker (executive, manager, analyst, etc.) to make better and faster decisions. The architecture of a DW exhibits various layers of data in which data from one layer are derived from data of the lower layer (see Figure 1). The operational databases, also called data sources, form the starting layer. They may c...

Extraction, Transformation, and Loading Processes

ETL stands for extraction, transformation, and loading, in other words, for the data warehouse (DW) backstage. The main focus of our exposition here is the practical application of the ETL process in real world cases with extra problems and strong requirements, particularly performance issues related to population of large data warehouses. In a context of ETL/DW with strong requirements, we can...

Distinct Peptide Loading Pathways for MHC